Breeding Programs

Outbred or Random Stocks

The terms ‘random’ and ‘outbred’ are often used interchangeably. At the ARC we use a randomisation table for outbred colonies less than 100 breeding females. For larger colonies future breeders are chosen from a maximum of one animal per cage. Several outbreeding systems are recognised but the common aim of these systems is a population represented by as large a range of alleles of each gene as possible, resulting in an inbreeding coefficient of no greater than 2% per generation.

Inbred Strains

Inbred strains are bred within a closed colony by brother-sister mating to maintain certain defining characteristics. Animals are considered inbred after at least 20 consecutive generations of brother-sister mating.

Substrain: Established inbred strains may genetically diverge with time into substrains if separated from the parental line for 20 or more generations.

Congenic Strains

A congenic strain is genetically identical to an inbred strain except for a short chromosomal segment bearing the gene of interest. The formation of a congenic strain begins with the crossing of two breeding groups. One provides the genetic background and must always be an inbred strain. The other ‘donates’ the gene of interest and may or may not be an inbred strain. The mating system that produces congenic strains depends upon whether the gene of interest is dominant or recessive and the gene’s effect, when homozygous, on reproductive performance. Generally, ten backcross generations are required to yield a congenic animal, although contemporary molecular techniques can significantly shorten this period. Once established, a congenic strain is managed identically to an inbred strain.

Hybrid Strains

A hybrid is the first generation progeny (F1) of parents from two different inbred strains. Genetic uniformity and hybrid vigour are two desirable features of hybrids. They are typically not bred, except on demand, as the unique features cannot be maintained beyond the F1 generation. Hybrid nomenclature places the female strain first and the name is commonly shortened.  For example the C57BL/6J (Female) x DBA/2 (Male) = B6D2F1, and C57BL (Female) x BALB/c (Male) = B6CF1.

Mutant Strains

As the name implies these animals display a feature of a genetic mutation. It may be as simple and benign as a coat-colour mutation or a complex mutation where animals are sexually infertile or predisposed to a disease state or death. In most cases the mutation can be transferred to both outbred and inbred animals. Mutations can be either spontaneous (e.g. the nude mutation Foxn1nu/Foxn1nu, and hairless mutation hr/hr) or induced (transgenic and knockout strains).